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In enzymology, an acyl-CoA oxidase (EC 1.3.3.6) is an enzyme that catalyzes the chemical reaction
acyl-CoA + O2 \rightleftharpoons trans-2,3-dehydroacyl-CoA + H2O2
Thus, the two substrates of this enzyme are acyl-CoA and O2, wheras its two products are trans-2,3-dehydroacyl-CoA and H2O2.