Cat No.
DIA-134
Description
Sterol esterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is steryl-ester acylhydrolase. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Esterase (Pseudomonas sp.)
Species
Pseudomonas sp.
Enzyme Commission Number
EC 3.1.1.13
Bio-activity
GradeIII 100U/mg-solid or more (containing approx. 40% of stabilizers)
Appearance
Light brown amorphous powder, lyophilized
Molecular Mass
approx. 300 kDa
pH Stability
pH 5.0-9.0 (25°C, 24hr)
Michaelis Constant
5.4×10??M (Linoleate), 6.6×10??M (Oleate), 3.7×10??M (Linolenate), 1.5×10??M (Palmitate), 1.2×10??M (Myristate), 2.3×10??M (Stearate)
Isoelectric point
5.9±0.1
Optimum pH
7.0-9.0
Optimum temperature
40°C
Stabilizers
Mg??, Na-cholate, bovine serum albumin
Thermal stability
below 55°C (pH 7.5, 10min)
Stability
Stable at-20°C for at least one year
Inhibitors
Hg??, Ag?, ionic detergents
Applications
This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Synonyms
cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; EC 3.1.1.13; Sterol esterase
