Cat No.
DIA-408
Description
Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.
Abbr
CHOD, Recombinant (E. coli)
Alias
CHOD
Source
E. coli
Species
E. coli
Form
Liquid form (30% sucrose solution)
Enzyme Commission Number
EC 1.1.3.6
Bio-activity
> 200 U/ml
Molecular Mass
ca. 59 kDa
pH Stability
3.5–8.5
Michaelis Constant
1.9 x 10^-5 M (cholesterol)
Structure
monomer of 60 kDa (SDS-PAGE)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of hydrogen peroxide per min at 37°C and pH 7.0.
Optimum pH
6.5–8.0
Optimum temperature
55°C–65°C
Thermal stability
below 70°C
Stability
stable at 25°C for at least one month
Inhibitors
Ag+, Hg2+
Synonyms
EC 1.1.3.6, cholesterol-O2 oxidoreductase; 3β-hydroxy steroid oxidoreductase; 3β-hydroxysteroid:oxygen oxidoreductase; 9028-76-6
