Cat No.
DIA-284
Description
Oxidoreductase that catalyzes the interconversion of cholesterol to cholest-4-en-3-one. Rely on the proven diagnostic quality of this product.
Abbr
Chemically modified CHOD, Recombinant (Brevibacterium sp.)
Alias
CHOD
Species
Brevibacterium species
Bio-activity
10-20 U/mg lyophilizate
Appearance
Yellow lyophilizate
Molecular Mass
60 kD (native and SDS)
pH Stability
5.0-10.0
Michaelis Constant
Cholesterol: 1 x 10-4 mol/l
Isoelectric point
~5.0
Specificity
cholesterol 100%
pregnenolon 52%
stigmasterol 17%
dehydroandrosterone 0.5%
androsterone 0%
estradiol 0%
cholate 0%
Optimum pH
5.5-8.0
Activators
Non ionic detergents
Thermal stability
Up to +55°C
Stability
At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.
Inhibitors
Hg2+, ZnCl2, SDS
Applications
Use Cholesterol Oxidase in diagnostic tests for the determination of cholesterol in combination with Cholesterol Esterase.
Synonyms
Cholesterol-O2 oxidoreductase; 3 beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid: oxygen oxidoreductase; cholesterol: oxygen oxidoreductase; cholesterol oxidase
