Cat No.
DIA-136
Description
Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Oxidase (Pseudomonas sp.)
Species
Pseudomonas sp.
Enzyme Commission Number
EC 1.1.3.6
Bio-activity
Grade Ⅲ 15 U/mg-solid or more(contg. approx. 40% of stabilizers)
CAS No.
9026-00-0
Reaction
cholesterol + O2 = cholest-4-en-3-one + H2O2
Appearance
Yellowish amorphous powder, lyophilized
Molecular Mass
about 34 kDa (gel filtration)
pH Stability
pH 5.0~10.0 (25°C, 20hr)
Michaelis Constant
2.1×10-2 M (Creatine)
Structure
dimer
Isoelectric point
5.1±0.1 and 5.4±0.1
Optimum pH
6.5~7.0
Optimum temperature
45~50°C
Thermal stability
below 45°C (pH 7.0, 15min)
Stability
Stable at-20°C for at least 6 months
Inhibitors
onic detergents, Hg2＋, Ag
Synonyms
Cholesterol-O2 oxidoreductase; 3 beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid: oxygen oxidoreductase; cholesterol: oxygen oxidoreductase; cholesterol oxidase; EC 1.1.3.6
