Cat No.
DIA-204
Description
In enzymology, a 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) is an enzyme that catalyzes the chemical reaction: (R)-3-hydroxybutanoate + NAD+ ? acetoacetate + NADH + H+. Thus, the two substrates of this enzyme are (R)-3-hydroxybutanoate and NAD+, whereas its three products are acetoacetate, NADH, and H+. This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in synthesis and degradation of ketone bodies and butanoate metabolism.
Abbr
D-3-hydroxybutyrate dehydrogenase (Pseudomonas sp.)
Species
Pseudomonas sp.
Enzyme Commission Number
EC 1.1.1.30
Bio-activity
GradeⅢ 100U/mg-solid or more
Appearance
White amorphous powder, lyophilized
Molecular Mass
approx. 130 kDa (by gel filtration)
pH Stability
pH 5.0-8.5 (25°C, 20hr)
Michaelis Constant
4.2×10??M (25°C, pH8.3), 7.0×10??M(37°C, pH8.3)(D-3-Hydroxybutyrate) 4.9×10??M (25°C, pH8.3), 7.2×10??M (37°C, pH8.3)(NAD?) 8.1×10??M (25°C, pH7.1), 2.4×10??M (37°C, pH7.1)(Acetoacetate) 8.4×10??M (25°C, pH7.1), 1.5×10??M (37°C, pH7.1)(NADH)
Isoelectric point
5.6±0.1
Optimum pH
8.3
Optimum temperature
55°C
Stabilizers
Sucrose, mannitol, bovine serum albumin
Thermal stability
below 40°C (pH 6.5, 15min)
Stability
Stable at-20°C for at least one year
Inhibitors
PCMB, MIA, IAA, Ag?, Hg??, SDS, DAC
Applications
This enzyme is useful for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.
Synonyms
(R)-3-hydroxybutanoate: NAD+ oxidoreductase; NAD+-beta-hydroxybutyrate dehydrogenase; hydroxybutyrate oxidoreductase; beta-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; D-3-hydroxybutyrate dehydrogenase; D-(–)-3-hydroxybutyrate dehydrogenase; beta-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyrate dehydrogenase; beta-hydroxybutyric dehydrogenase; EC 1.1.1.30
