Cat No.
DIA-320
Abbr
Diaphorase, Recombinant (E. coli)
Species
E. coli
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 1.8.1.4
Bio-activity
12 U/mg protein at pH 9.0 and 25°C.
Molecular Mass
~ 51,700
Isoelectric point
~ 6.0
Unit Definition
One Unit of diaphorase is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH.
Optimum pH
9
Thermal stability
up to 25°C
Stability
> 2 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in 0.5 mM FAD containing 0.5 mg/ml BSA. Swirl to mix the enzyme suspension immediately prior to use.
Applications
High purity Diaphorase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
dehydrolipoate dehydrogenase; diaphorase; dihydrolipoamide dehydrogenase; dihydrolipoamide:NAD+ oxidoreductase; dihydrolipoic dehydrogenase; dihydrothioctic dehydrogenase; lipoamide dehydrogenase (NADH); lipoamide oxidoreductase (NADH); lipoamide reductase; lipoamide reductase (NADH); lipoate dehydrogenase; lipoic acid dehydrogenase; lipoyl dehydrogenase; dihydrolipoyl dehydrogenase; EC 1.8.1.4; 9001-18-7; Lipoamide Dehydrogenase
