Cat No.
DIA-187
Abbr
Diaphorase (Clostridium sp.)
Alias
Diaphorase
Species
Clostridium sp.
Form
Freeze dried powder
Enzyme Commission Number
EC 1.6.99.-
Bio-activity
GradeⅢ 30U/mg-solid or more (containing approx. 15% of stabilizers)
Appearance
Yellowish amorphous powder, lyophilized
Molecular Mass
24 kDa
pH Stability
pH 7.5 (30°C, 3hr)
Michaelis Constant
2.0×10??M (NADH), 6.0×10??M (NADPH)
Structure
One mol of FMN per mol of enzyme
Specificity
Either NADH or NADPH can be used as a reductant. The catalytic ratio (NADPH/NADH) is 0.6 in the assay method. Neither oxygen nor cytochrome C can be utilized as a hydrogen acceptor.
Optimum pH
8.5
Optimum temperature
50°C
Stabilizers
FMN, NAD(P)H
Thermal stability
below 30°C (pH 7.5, 30min)
Stability
Stable at-20°C for at least one year
Inhibitors
N-Ethylmaleimide
Applications
This enzyme is useful for colorimetric determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H.
Synonyms
Diaphorase; EC 1.6.99.-
