Cat No.
DIA-145
Description
Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) (EC 1.1.1.49) is a cytosolic enzyme that catalyzes the chemical reaction:D-glucose 6-phosphate + NADP+ ? 6-phospho-D-glucono-1,5-lactone + NADPH + H+. This enzyme is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).
Abbr
G6PDH (Microorganism)
Alias
G6PDH; GPD; G6PD1; G6PD
Species
Microorganism
Form
Freeze dried powder
Enzyme Commission Number
EC 1.1.1.49
Bio-activity
GradeⅢ 200U/mg-solid or more
Appearance
White amorphous powder, lyophilized
Molecular Mass
approx. 140 kDa (by gel filtration)
pH Stability
pH 5.0-11.0 (25°C, 22hr)
Michaelis Constant
NAD? linked 2.4×10??M (NAD?), 4.7×10??M (G-6-P), NADP? linked 7.4×10??M (NADP?), 3.2×10??M (G-6-P)
Optimum pH
7.8
Optimum temperature
50°C-55°C
Thermal stability
below 50°C (pH 7.8, 30min)
Stability
Stable at-20°C for at least one year
Inhibitors
Metal ions, iodoacetamimide, SDS etc.
Applications
The enzyme is useful for enzymatic determination of NAD+(NADP+) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase. The enzyme is also used for enzymatic determination of glucose and creatine phosphokinase activity when coupled with hexokinase.
Synonyms
Glucose-6-phosphate dehydrogenase; G6PD; G6PDH; Glucose-6-phosphate dehydrogenase (NADP(+)); EC 1.1.1.49; Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate dehydrogenase; GPD
