Cat No.
DIA-411
Description
Glutamine synthetase (GS) (EC 6.3.1.2) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine: Glutamate + ATP + NH3 → Glutamine + ADP + phosphate. Glutamine Synthetase uses ammonia produced by nitrate reduction, amino acid degradation, and photorespiration. The amide group of glutamate is a nitrogen source for the synthesis of glutamine pathway metabolites.
Abbr
GS, Native (Microorganism)
Alias
GS
Species
Microorganism
Enzyme Commission Number
EC 6.3.1.2
Bio-activity
> 7 U/mg lyophilizate
Appearance
Light yellow lyophilizate
Molecular Mass
ca. 900 kDa
pH Stability
6.5–9.5
Michaelis Constant
1.5 x 10^-2 M (L-glutamate)
1.3 x 10^-4 M (ammonia)
8.7 x 10^-4 M (ATP)
Structure
57 kDa (SDS-PAGE)
Isoelectric point
6.5
Specificity
L-glutamate (100), D-glutamate (0.8), NH3 (100), NH2OH (12), ATP (100), GTP (2.5)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of phosphate per min at 37°C and pH 7.0.
Optimum pH
7
Optimum temperature
60°C
Stabilizers
Sucrose
Activators
Mg2+, Mn2+
Thermal stability
below 40°C
Inhibitors
methionine sulfoximine, carbamyl phosphate
Synonyms
Glutamine synthetase; GS; EC 6.3.1.2; Glutamate-ammonia ligase
