Cat No.
DIA-202
Description
A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose.
Abbr
Hexokinase (Microorganism)
Alias
Hexokinase
Species
Microorganism
Enzyme Commission Number
EC 2.7.1.1
Bio-activity
GradeⅢ 150U/mg-solid or more
Reaction
Hexose-CH2OH + MgATP 2-→ Hexose-CH2O-PO3 2-+ MgADP-+ H+
Appearance
White amorphous powder, lyophilized
Molecular Mass
approx. 82 kDa (by gel filtration)
pH Stability
pH 4.0-9.0 (25°C, 20hr)
Michaelis Constant
2.3×10??M (D-Glucose), 7.7×10??M (ATP)
Isoelectric point
4.1±0.1
Optimum pH
8.0-9.0
Optimum temperature
50°C
Thermal stability
below 45°C (pH 7.0, 30min)
Stability
Store at-20°
Inhibitors
Metal ions, p-chloromercuribenzoate, iodoacetamide, SDS, etc
Applications
The enzyme is useful for enzymatic determination of glucose, adenosine-5'-triphosphate (ATP) and creatine phosphokinase when coupled with glucose-6-phosphate dehydrogenase.
Synonyms
Hexokinase; EC 2.7.1.1; hexokinase type IV glucokinase; hexokinase D; hexokinase type IV; hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; ATP: D-hexose 6-phosphotransferase
