Cat No.
DIA-412
Description
Histamine dehydrogenase is a homodimeric enzyme and catalyzes oxidative deamination of histamine. The gene encoding this enzyme has been sequenced and cloned by polymerase chain reactions and overexpressed in Escherichia coli.
Abbr
Histamine Dehydrogenase, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Enzyme Commission Number
EC 1.4.99
Bio-activity
> 1 U/mg lyophilizate
Appearance
Brownish lyophilizate
Molecular Mass
ca. 150 kDa
pH Stability
5.5–10.5
Michaelis Constant
1.0 x 10^-5 M (histamine)
Structure
2 subunits of ca. 71 kDa (SDS-PAGE)
Specificity
histamine (100), agmatine (7), putrescine (0), cadaverine (0)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of 4-imidazolylacetoaldehyde per min at 37°C and pH 9.0.
Optimum pH
9.0–9.5
Optimum temperature
70°C
Stabilizers
Trehalose
Thermal stability
below 65°C
Stability
Stability (liquid form) stable at 37°C for at least four weeks
Stability (powder form) stable at 50°C for at least two weeks
Synonyms
Histamine : (acceptor) oxidoreductase (deaminating); EC 1.4.99; Histamine Dehydrogenase
