Cat No.
DIA-283
Description
Oxidoreductase that oxidizes ascorbic acid to dehydroascorbate. Take advantage of the improved stability in liquid reagents. Rely on the proven diagnostic quality of this product.
Abbr
Chemically modified AAO (Cucurbita sp.)
Alias
AAO
Species
Cucurbita species
Bio-activity
>180 U/mg lyophilizate (+37°C, L-ascorbate); Specific activity (+37°C): >1,800 U/mg protein
Appearance
Turquoise lyophilizate
Molecular Mass
Approximately 140 kD
pH Stability
6.5-9.0
Michaelis Constant
L-ascorbate: 3 x 10-4 mol/l
Isoelectric point
5.0-6.0
Specificity
Several analogs of ascorbate react.
Optimum pH
5.6-7.0
Thermal stability
Up to +70°C
Stability
At -15 to -25°C within specification range for 12 months. Store dry. Keep tightly sealed.
Inhibitors
4-chloromercuribenzoate, CN-, Na2S, diethyl-dithiocarbamate, 8-hydroxyquinoline, K-ethylxanthate
Applications
Use Ascorbate Oxidase, chemically modified, in a variety of diagnostic tests to eliminate the interference of ascorbic acid, since ascorbic acid interferes with the Trinder reaction that is widely used for the colorimetric determination of analytes. It is useful in liquid as well as dry chemistry test, e.g., for the determination of uric acid, lactate or creatinine.
Synonyms
ascorbase; ascorbic acid oxidase; ascorbate oxidase; ascorbic oxidase; ascorbate dehydrogenase; L-ascorbic acid oxidase; AAO; L-ascorbate: O2 oxidoreductase; AA oxidase; L-ascorbate oxidase
