Cat No.
DIA-200
Description
In enzymology, a glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the chemical reaction: sn-glycerol 3-phosphate + O2 ? glycerone phosphate + H2O2. Thus, the two substrates of this enzyme are sn-glycerol 3-phosphate and O2, whereas its two products are glycerone phosphate and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycerophospholipid metabolism. It employs one cofactor, FAD.
Abbr
L-α-glycerophosphate oxidase (Microorganism)
Species
Microorganism
Enzyme Commission Number
EC 1.1.3.21
Bio-activity
GradeⅢ 15 U/mg-solid or more (containing approx. 60% of stabilizers)
Appearance
Yellowish amorphous powder, lyophilized
Molecular Mass
approx. 93 kDa (by gel filtration)
pH Stability
5.0-7.5 (25°C, 60min)
Michaelis Constant
2.3×10?3M (L-α-Glycerophosphate)
Isoelectric point
4.6±0.1
Optimum pH
6.5-7.0
Optimum temperature
40°C
Stabilizers
Sucrose, FAD
Thermal stability
below 45°C (pH 6.5, 10min)
Stability
Stable at-20°C for at least 6 months
Inhibitors
SH-reagents, ionic detergents, metal ions, etc.
Applications
This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase and glycerokinase in clinical analysis.
Synonyms
L-α-glycerophosphate oxidase; sn-glycerol-3-phosphate: oxygen 2-oxidoreductase; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-alpha-glycerophosphate oxidase; alpha-glycerophosphate oxidase; L-alpha-glycerol-3-phosphate oxidase; EC 1.1.3.21
