Cat No.
DIA-278
Description
Dehydrogenase that catalyzes the interconversion of malate to oxaloacetate. Rely on the proven diagnostic quality of this product. Tested according to the recommendations of the International Federation of Clinical Chemistry (IFCC).
Abbr
MDH, Native (Porcine) (IFCC Quality)
Alias
MDH
Source
Porcine heart
Species
Porcine
Bio-activity
>70 U/mg lyophilizate
Appearance
White lyophilizate
Molecular Mass
70 kDa
pH Stability
7.0-9.0
Michaelis Constant
L-malate: 4.0 x 10-4 mol/l
L-tartrate: 9.0 x 10-3 mol/l
meso-tartrate: 1.2 x 10-3 mol/l
oxaloacetate: 3.3 x 10-5 mol/l
Isoelectric point
6.1-6.4
Specificity
L-configuration of malate and tartrate. NAD can be replaced by its analogs, but not by NADP.
Optimum pH
7.5
Activators
Phosphate, arsenate, Zn2+
Thermal stability
Up to +40°C
Stability
At +2 to +8°C within specification range for 12 months. Store dry.
Inhibitors
Iodinated compounds such as iodine cyanide, thyroxine and molecular iodine, phenols, 1,10-phenanthroline, 8-hydroxyquinoline, sulfide, nicotinic acidamide, adenine, AMP, ATP; oxaloacetate (excess).
Applications
Use Malate Dehydrogenase in diagnostic tests for the determination of aspartate aminotransferase or in applications for citric and acetic acid testing.
Synonyms
Malate Dehydrogenase, IFCC Quality; malic dehydrogenase; L-malate dehydrogenase; malic acid dehydrogenase; MDH
