Cat No.
DIA-182
Description
In enzymology, a N-acetylneuraminate lyase (EC 4.1.3.3) is an enzyme that catalyzes the chemical reaction: N-acetylneuraminate ? N-acetyl-D-mannosamine + pyruvate. Hence, this enzyme has one substrate, N-acetylneuraminate, and two products, N-acetyl-D-mannosamine and pyruvate. This enzyme belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds.
Abbr
NALase (Microorganism)
Alias
NPL; NALase; NANA lyase
Species
Microorganism
Form
Freeze dried powder
Enzyme Commission Number
EC 4.1.3.3
Bio-activity
Grade III 15U/mg-solid or more (30U/mg-protein or more), (containing approx. 30% of stabilizers)
Appearance
Yellowish amorphous powder, lyophilized
Molecular Mass
approx. 98 kDa
pH Stability
pH 6.0?9.0 (10°C, 25hr)
Michaelis Constant
2.5×10?3M (N-Acetylneuraminic acid)
Structure
3 subunits (approx. 35,000) per mol of enzyme
Isoelectric point
4.6±0.1
Optimum pH
7.5-8.0
Optimum temperature
70°C
Thermal stability
below 65°C (pH 7.5, 30min)
Stability
Stable at-20°C for at least 6 months
Inhibitors
p-Chloromercuribenzoate, sodium dodecyl sulfate, Hg??, Ag?
Applications
This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when coupled with the related enzymes in clinical analysis. For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
Synonyms
N-Acetylneuraminate Pyruvate Lyase; N-Acetylneuraminic Acid Lyase; NANA Aldolase; EC 4.1.3.3; N-acetylneuraminate pyruvate-lyase (N-acetyl-D-mannosamine-forming); N-acetylneuraminic acid aldolase; acetylneuraminate lyase; sialic aldolase; sialic acid aldolase; sialate lyase; N-acetylneuraminic aldolase; neuraminic aldolase; N-acetylneuraminate aldolase; neuraminic acid aldolase; N-acetylneuraminic acid aldolase; neuraminate aldolase; N-acetylneuraminic lyase; NPL; NALase; NANA lyase; acetylneuraminate pyruvate-lyase; N-acetylneuraminate pyruvate-lyase
