Cat No.
DIA-213
Description
Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in humans is encoded by the PREP gene.
Abbr
PE (Flavobacterium sp.)
Alias
PE
Species
Flavobacterium sp.
Enzyme Commission Number
EC 3.4.21.26
Bio-activity
GradeⅠ 5.0U/mg-solid or more
Appearance
White amorphous powder, lyophilized
Molecular Mass
approx. 78 kDa
pH Stability
5.5-8.5 (30°C,15hr)
Michaelis Constant
2.5×10??M (Z-Gly-Pro-MCA), 1.4×10??M (Z-Gly-Pro-2NNap)
Structure
Monomer
Isoelectric point
9.1
Specificity
Y-Pro(Ala)-X (Y, peptide or N-protected amino acid; X, amino acid, peptide, amide, or ester)
Optimum pH
6.5
Optimum temperature
37°C (40°C)
Thermal stability
below 40°C (pH 7.0,10 min)
Stability
Stable at-20°C
Inhibitors
DFP, 3, 4-dichloroisocoumarin, Z-Gly-Pro-CH?Cl
Applications
This enzyme is useful for the determination of amino acid sequences of peptides and proteins containing proline residues.
Synonyms
EC 3.4.21.26; post-proline cleaving enzyme; proline-specific endopeptidase; post-proline endopeptidase; proline endopeptidase; endoprolylpeptidase; prolyl endopeptidase; prolyl oligopeptidase; PE
