Cat No.
DIA-164
Description
Purine nucleoside phosphorylase (also known as PNPase) is an enzyme (EC 2.4.2.1) involved in purine metabolism. PNP metabolizes adenosine into adenine, inosine into hypoxanthine, and guanosine into guanine, in each case creating ribose phosphate. NP encodes the enzyme purine nucleoside phosphorylase that together with adenosine deaminase (ADA) serves a key role in purine catabolism, referred to as the salvage pathway. Mutations in either enzyme result in a severe combined immunodeficiency (SCID). Confusingly, the same abbreviation (PNPase), is also used for another, otherwise unrelated, enzyme, namely Polynucleotide Phosphorylase.
Abbr
PNPase (Bacillus sp.)
Alias
PNPase; PUNPI; PUNPII
Species
Bacillus sp.
Form
Liquid
Enzyme Commission Number
EC 2.4.2.1
Bio-activity
> 500U/mL
CAS No.
9030-21-1
Reaction
purine nucleoside + phosphate = purine + α-D-ribose 1-phosphate
Appearance
Colourless to light brown solution
pH Stability
6.0-10.0 (37°C (Tris-HCI buffer)
Optimum pH
8
Thermal stability
Stable at 65°C and below (pH 8.5, 10 mins)
Inhibitors
Ag+, Hg2+
Applications
Useful for enzymatic determination of inorganic phosphate
Gene Name
NP(Homo sapiens)
Synonyms
inosine phosphorylase; PNPase; PUNPI; PUNPII; inosine-guanosine phosphorylase; nucleotide phosphatase; purine deoxynucleoside phosphorylase; purine deoxyribonucleoside phosphorylase; purine nucleoside phosphorylase; purine ribonucleoside phosphorylase; purine-nucleoside: phosphate ribosyltransferase; EC 2.4.2.1
GeneID
4860
mRNA Refseq
NM_000270
Protein Refseq
NP_000261
MIM
164050
UniProt ID
P00491
Chromosome Location
14q13.1
Pathway
Nicotinate and nicotinamide metabolism; Purine metabolism; Pyrimidine metabolism; Nucleotide metabolism.
Function
purine-nucleoside phosphorylase activity; purine-nucleoside phosphorylase activity; purine-nucleoside phosphorylase activity.
