Cat No.
DIA-171
Description
Sarcosine oxidase (SAO) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2, 5, 10-CH2-tetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen. sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.
Abbr
SAO (Microorganism)
Alias
SAO
Species
Microorganism
Form
Freeze dried powder
Enzyme Commission Number
EC 1.5.3.1
Bio-activity
GradeⅢ 8.0U/mg-solid or more
CAS No.
9029-22-5
Appearance
Yellowish amorphous powder, lyophilized
Molecular Mass
approx. 65 kDa (by gel filtration)
pH Stability
pH 6.5-9.0 (25°C, 24hr)
Michaelis Constant
2.8×10?3M (Sarcosine)
Isoelectric point
4.9±0.1
Optimum pH
7.0-8.5
Optimum temperature
40-50°C
Stabilizers
Potassium gluconate
Thermal stability
below 55°C (pH 7.5, 10min)
Stability
Stable at-20°C for at least one year
Inhibitors
Cu??, Ag?, Hg??, p-chloromercuribenzoate, N-ethylmaleimide, SDS
Applications
This enzyme is useful for enzymatic determination of creatinine, creatine, and sarcosine when coupling with creatinine amidohydrolase and creatine amidinohydrolase.-341 is newer type of sarosine oxidase, with improved stability in antimicrobial reagent.
Gene Name
SAO (Bos taurus)
Synonyms
Sarcosine Oxidase; EC 1.5.3.1; SAO
GeneID
789307
mRNA Refseq
NM_001130764
Protein Refseq
NP_001124236
UniProt ID
Q29437
Chromosome Location
19
Function
Amine oxidase activity; oxidoreductase activity; calcium ion binding; copper ion binding; quinone binding.
