Cat No.
DIA-404
Description
The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2
Abbr
UO, Recombinant (Candida utilis)
Source
Escherichia coli
Species
Candida utilis
Enzyme Commission Number
EC 1.7.3.3
Bio-activity
> 6 U/mg
Appearance
White to cream powder
Molecular Mass
34kDa (SDS-PAGE)
pH Stability
7.6 to 10.0
Unit Definition
One unit of activity is defined as the amount of enzyme that will transform of 1.0 micromole of substrate per minute at 25°C under standard assay method conditions.
Optimum pH
8.5
Optimum temperature
55°C
Thermal stability
Stable at 55°C and below.
Applications
Used in the enzymatic determination of uric acid.
Synonyms
urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II
