Cat No.
DIA-218
Description
Xanthine oxidase is a form of xanthine oxidoreductase, a type of enzyme that generates reactive oxygen species. These enzymes catalyze the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. These enzymes play an important role in the catabolism of purines in some species, including humans.
Abbr
XAO (Microorganism)
Alias
XO; XAO
Species
Microorganism
Enzyme Commission Number
EC 1.1.3.22
Bio-activity
GradeⅡ 10U/mg-solid or more
Appearance
Reddish brown amorphous powder, lyophilized
Molecular Mass
approx. 160 kDa
pH Stability
pH 6.5-9.0 (25°C, 15hr)
Michaelis Constant
4.5×10??M (Xanthine), 7.6×10??M (Hypoxanthine)
Isoelectric point
4.0±0.1
Optimum pH
7.5-8.0
Optimum temperature
65°C
Stabilizers
Sodium glutamate, BSA
Thermal stability
below 55°C (pH 8.0, 30min)
Stability
Stable at-20°C for at least year
Inhibitors
Reducing agents, Hg??, Ag?,MIA
Applications
This enzyme is useful for enzymatic determination of inorganic phosphorus, 5'-nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase and uricase.
Synonyms
EC 1.1.3.22; Xanthine oxidase; XO; XAO
