Cat No.
NATE-1099
Description
In enzymology, a 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) is an enzyme that catalyzes the chemical reaction: (R)-3-hydroxybutanoate + NAD+ ? acetoacetate + NADH + H+. Thus, the two substrates of this enzyme are (R)-3 hydroxybutanoate and NAD+, whereas its three products are acetoacetate, NADH, and H+. This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Abbr
3-HBDH, Recombinant (Prokaryote)
Alias
3-HBDH
Species
Prokaryote
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 1.1.1.30
Bio-activity
140 U/mg protein at pH 8.0 and 25°C.
Molecular Mass
~ 29,177
Isoelectric point
~ 6.9
Unit Definition
One Unit of 3-hydroxybutyrate dehydrogenase (3-HBDH) is defined as the amount of enzyme required to produce one μmole of NADH from NAD+.
Optimum pH
8
Thermal stability
up to 25°C
Stability
> 2 years at 4°C
Preparation Instructions
For assay, this enzyme should be diluted in 200 mM Tris/HCl buffer, pH 8.0. Swirl to mix the enzyme suspension immediately prior to use.
Applications
High purity recombinant 3-Hydroxybutyrate dehydrogenase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
3-hydroxybutyrate dehydrogenase; 3-HBDH; NAD-β-hydroxybutyrate dehydrogenase; hydroxybutyrate oxidoreductase; β-hydroxybutyrate dehydrogenase; D-β-hydroxybutyrate dehydrogenase; D-3-hydroxybutyrate dehydrogenase; D- (–)-3-hydroxybutyrate dehydrogenase; β-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyrate dehydrogenase; β-hydroxybutyric dehydrogenase; EC 1.1.1.30; 9028-38-0
