Cat No.
NATE-1167
Description
In enzymology, a phosphogluconate dehydrogenase (decarboxylating) (EC 1.1.1.44) is an enzyme that catalyzes the chemical reaction:6-phospho-D-gluconate + NADP+? D-ribulose 5-phosphate + CO2 + NADPH. Thus, the two substRates of this enzyme are 6-phospho-D-gluconate and NADP+, whereas its 3 products are D-ribulose 5-phosphate, CO2, and NADPH. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Abbr
6-Phosphogluconic Dehydrogenase, Native (E. coli)
Species
E. coli
Form
Suspension in Ammonium Sulphate
Enzyme Commission Number
EC 1.1.1.44
Bio-activity
> 150 UI/ml, > 45 U/mg
Unit Definition
One Units of 6-phospho-D-gluconate dehydrogenase is defined as the amount of enzyme required to produce one μmole of NADPH from NADP+ in a coupled assay with gluconate kinase.
Optimum pH
7.5
Optimum temperature
55 °C
Applications
Determination of D-Gluconate and D-Glucono-δ-lactone in foodstaffs.
Synonyms
6-Phosphogluconic Dehydrogenase; phosphogluconic acid dehydrogenase; 6-phosphogluconic dehydrogenase; 6-phosphogluconic carboxylase; 6-phosphogluconate dehydrogenase (decarboxylating); 6-phospho-D-gluconate dehydrogenase; EC 1.1.1.44; phosphogluconate dehydrogenase; decarboxylating; 9073-95-4
