Cat No.
NATE-0796
Description
In enzymology, a phosphogluconate dehydrogenase (decarboxylating) (EC 1.1.1.44) is an enzyme that catalyzes the chemical reaction:6-phospho-D-gluconate + NADP+? D-ribulose 5-phosphate + CO2 + NADPH. Thus, the two substRates of this enzyme are 6-phospho-D-gluconate and NADP+, whereas its 3 products are D-ribulose 5-phosphate, CO2, and NADPH. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Abbr
6-Phosphogluconate dehydrogenase, Recombinant (E. coli)
Species
E. coli
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 1.1.1.44
Bio-activity
38 U/mg protein at pH 7.6 and 25°C.
Molecular Mass
~ 52,546 Da
Isoelectric point
~ 5.3
Unit Definition
One Unit of 6-phosphogluconate dehydrogenase (6-PGDH) is defined as the amount of enzyme required to produce one μmole of NADPH from NADP+.
Optimum pH
7.6
Optimum temperature
25°C
Stability
> 2 years at 4°C.
Applications
High purity recombinant 6-Phosphogluconate dehydrogenase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
6-Phosphogluconic Dehydrogenase; phosphogluconic acid dehydrogenase; 6-phosphogluconic dehydrogenase; 6-phosphogluconic carboxylase; 6-phosphogluconate dehydrogenase (decarboxylating); 6-phospho-D-gluconate dehydrogenase; EC 1.1.1.44; phosphogluconate dehydrogenase; decarboxylating
