Cat No.
NATE-0864
Description
In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction:2 L-ascorbate + O2? 2 dehydroascorbate + 2 H2O. Thus, the two substRates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O. This enzyme belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. This enzyme participates in ascorbate metabolism. It employs one cofactor, copper.
Abbr
AAO, Native (Acremonium sp.)
Alias
AAO; ascorbase
Species
Acremonium sp.
Form
Freeze dried powder
Enzyme Commission Number
EC 1.10.3.3
Bio-activity
> 200 U/mg
Appearance
Light blue amorphous powder, lyophilized
Molecular Mass
80 kDa（gel filtration）
pH Stability
6.0?10.0（30°C, 24 hr）
Michaelis Constant
Ascorbic acid（pH 7.0） 1.0 × 10-4 M
Ascorbic acid（pH 4.0） 3.8 × 10-4 M
Isoelectric point
4
Optimum pH
4.0?4.5
Stabilizers
BSA, Mannitol
Thermal stability
Stable at 50°C and below (pH 7.0, 10 mins)
Applications
This enzyme is useful for avoidance from interference of ascorbic acid on diagnostic assay such as blood, uric acid, TG, TC and creatinine.
Synonyms
ascorbase; ascorbic acid oxidase; ascorbate oxidase; ascorbic oxidase; ascorbate dehydrogenase; L-ascorbic acid oxidase; AAO; L-ascorbate:O2 oxidoreductase; AA oxidase; EC 1.10.3.3; L-ascorbate oxidase
