Cat No.
NATE-0039
Abbr
AG6PDHII, Native (E. coli)
Alias
AG6PDHII
Species
Escherichia coli
Form
Freeze dried powder
Enzyme Commission Number
EC 1.1.1.140
Bio-activity
> 20 U/mg
Appearance
White powder
Molecular Mass
78 kDa (TSK gel G 3000 SWXL gel filtration); 40 kDa (SDS?PAGE)
pH Stability
7.0-9.0 (50°C, 30 mins)
Michaelis Constant
1,5?Anhydroglucitol?6?phosphate 25 mM (pH 10.0); NADP 0.09 mM (pH 10.0)
Isoelectric point
pH 4.7
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of 1,5?AG?6?phosphate to C6H11O8P per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
9.0-10.0
Optimum temperature
37?50°C
Thermal stability
Stable at 42°C and below (pH 9.5, 30 mins)
Stability
Stable for 1 years under the freezing condition (-80°C).
Applications
Useful for enzymatic determination of 1,5-AG
Synonyms
AG6PDHII; EC 1.1.1.140; 1-5-Anhydroglucitol-6-Phosphate Dehydrogenase
