Cat No.
NATE-0046
Description
In enzymology, an alcohol oxidase (EC 1.1.3.13) is an enzyme that catalyzes the chemical reaction:a primary alcohol + O2? an aldehyde + H2O2. Thus, the two substrates of this enzyme are primary alcohol and O2, whereas its two products are aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. It employs one cofactor, FAD.
Abbr
Alcohol Oxidase, Native (Hansenula sp.)
Alias
alcohol oxidase; ethanol oxidase
Species
Hansenula sp.
Form
vacuum-dried powder
Enzyme Commission Number
EC 1.1.3.13
Bio-activity
> 0.6 units/mg solid
CAS No.
9073-63-6
Molecular Mass
~600 kDa
pH Stability
pH Range: 5.5-8.5
Unit Definition
One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25°C.
Optimum pH
8.5
Stability
?20°C
Inhibitors
1,4-butynediol (irreversible), propargyl alcohol (irreversible), cyclopropanol, cyclopropanone (suicide substrate), formaldehyde, H2O2, hydroxylamine, KBr, KCN, methanol (substrate inhibitor), NaN3, PCMB, propynal, urea, 4-chloromercuribenzoic acid
Applications
Alcohol oxidase is used to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It may be used to study methanol metabolism is yeasts, such as Candida, Pichia, and Hansenula. It is useful to study protein translocation into peroxisomes.
Synonyms
EC 1.1.3.13; 9073-63-6; alcohol oxidase; ethanol oxidase; Alcohol:oxygen oxidoreductase
