Cat No.
NATE-0892
Description
Cholesterol dehydrogenase is an enzyme that uses nicotinamide adenine dinucleotide/nicotinamide adenine dinucleotide phosphate (NAD(P)) as its cofactor in oxidizing cholesterol to form cholest-4-en-3-one. This enzyme oxidizes the hydroxyl group at the 3 position of the sterol ring to form a ketone.
Abbr
CDH, Native (Nocardia sp.)
Alias
CDH
Species
Nocardia sp.
Form
Freeze dried powder
Bio-activity
> 5 U/mg
Appearance
Light yellow to brown powder
Molecular Mass
37 kDa (SDS-PAGE)
pH Stability
6.5 - 7.5 (37°C, 15 mins)
Isoelectric point
4.5
Unit Definition
One unit of activity is defined as the amount of enzyme that will catalyse the production of 1.0 micromole of NADH per minute at 25°C under standard assay method conditions.
Optimum pH
10
Optimum temperature
30°C
Activators
Triton X-100
Thermal stability
Stable at 35°C and below (pH 7.0, 5 mins)
Inhibitors
Ag+
Applications
Used in the formulation of Cholesterol testing reagents or in biosensor applications.
Synonyms
Cholesterol Dehydrogenase; CDH
