Cat No.
NATE-0156
Description
Chloroperoxidase (CPO) is a 42 kDa Da extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group. CPO is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme. It also catalyzes peroxidase-, catalase-and cytochrome P450-type reactions of dehydrogenation, H2O2 decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs).
Abbr
CPO, Native (Caldariomyces fumago)
Alias
CPO
Species
Caldariomyces fumago
Form
buffered aqueous suspension. Purified suspension in 0.1 M sodium phosphate solution, pH approx. 4.5
Enzyme Commission Number
EC 1.11.1.10
Bio-activity
1,000-2,000 units/mg protein (E1%/280); > 3,000 units/mL; >10,000 U/mL
CAS No.
9055-20-3
Unit Definition
One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25°C in the presence of potassium chloride and H2O2.
Applications
A useful alternative to lactoperoxidase for 131I ion labeling studies, for bromination of proteins, and for Cl labeling of macromolecules in long-term isolation procedures.
Synonyms
Chloroperoxidase; CPO; Vanadium haloperoxidase; EC 1.11.1.10; 9055-20-3; Chloride Peroxidase; Chloride:hydrogen-peroxide oxidoreductase
