Cat No.
NATE-0188
Description
Diamine oxidase from porcine kidney is a homodimer consisting of 2 equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper. The molecular mass of the enzyme is found to be 170 kDa. The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A. It catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide. Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.2 The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism. It is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.
Abbr
Diamine Oxidase, Native (Plant origin)
Alias
Diamine Oxidase
Species
Plant origin
Form
Tan Liquid
Enzyme Commission Number
EC 1.4.3.6
CAS No.
9001-53-0
Unit Definition
1 IU = 1 μmole H2O2/min decrease at 37°C and pH 6.5 as measured in a coupled reaction with lactoperoxidase (LPO), Putracine, and the chromogen ABTS.
Applications
DAO catalyzes the oxidation of diamines (and some monoamines) to produce the aldehyde, ammonia, and H2O2.
Synonyms
EC 1.4.3.6; 9001-53-0; Amine:oxygen oxidoreductase (deaminating) (pyridoxal-containing); Diamine Oxidase; Amine oxidase (copper-containing)
