Cat No.
NATE-0840
Description
In enzymology, a galactose 1-dehydrogenase (EC 1.1.1.48) is an enzyme that catalyzes the chemical reaction: D-galactose + NAD+ rightleftharpoons D-galactono-1,4-lactone + NADH + H+. Thus, the two substrates of this enzyme are D-galactose and NAD+, whereas its 3 products are D-galactono-1,4-lactone, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in galactose metabolism.
Abbr
Galactose dehydrogenase, Recombinant (Soil prokaryote)
Species
Soil prokaryote
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 1.1.1.48
Bio-activity
278 U/mg protein at pH 8.6 and 25°C.
Molecular Mass
~ 36,659 Da
Isoelectric point
~ 6.0
Unit Definition
One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+.
Optimum pH
8.6
Optimum temperature
40°C
Stability
> 2 years at 4°C.
Applications
High purity recombinant Galactose dehydrogenase (soil prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
D-galactose:NAD+ 1-oxidoreductase; D-galactose dehydrogenase; beta-galactose dehydrogenase; NAD+-dependent D-galactose dehydrogenase; galactose 1-dehydrogenase; EC 1.1.1.48; Galactose dehydrogenase
