Cat No.
NATE-0751
Description
α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate.
Abbr
α-GDH-TPI, Native (Rabbit)
Alias
α-GDH-TPI
Source
Rabbit muscle
Species
Rabbit
Form
ammonium sulfate suspension. Mixed crystalline suspension in 3.2 M (NH4)2SO4, pH 6
Enzyme Commission Number
EC 1.1.1.8
Bio-activity
TPI 750-2000 units/mg protein, GDH 75-200 units/mg protein (biuret)
Unit Definition
(α-GDH) One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25°C. (TPI) One unit will convert 1.0 μmole of D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25°C.
Applications
α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay.
Synonyms
α-glycerol phosphate dehydrogenase (NAD); α-glycerophosphate dehydrogenase (NAD); glycerol 1-phosphate dehydrogenase; glycerol phosphate dehydrogenase (NAD); glycerophosphate dehydrogenase (NAD); hydroglycerophosphate dehydrogenase; L-α-glycerol phosphate dehydrogenase; L-α-glycerophosphate dehydrogenase; L-glycerol phosphate dehydrogenase; L-glycerophosphate dehydrogenase; NAD-α-glycerophosphate dehydrogenase; NAD-dependent glycerol phosphate dehydrogenase; NAD-dependent glycerol-3-phosphate dehydrogenase; NAD-L-glycerol-3-phosphate dehydrogenase; NAD-linked glycerol 3-phosphate dehydrogenase; NADH-dihydroxyacetone phosphate reductase; glycerol-3-phosphate dehydrogenase (NAD); EC 1.1.1.8; α-GDH-TPI
