Cat No.
NATE-0407
Description
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.
Abbr
Lipoxygenase, Native (Glycine max (soybean))
Species
Glycine max (soybean)
Form
Type I, ammonium sulfate suspension, Suspension in 2.3 M (NH4)2SO4 solution, pH approx. 6.0; Type II, lyophilized powder, Contains stabilizer and NaCl.
Enzyme Commission Number
EC 1.13.11.12
Bio-activity
Type I, 500,000-1,000,000 units/mg protein; Type II, > 50,000 units/mg solid.
CAS No.
9029-60-1
Molecular Mass
mol wt 108 kDa (two 54 kDa subunits)
Unit Definition
One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25°C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.
Applications
The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12-or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).
Warnings
Prepared by affinity chromatography. Protein determined by biuret.
Synonyms
Lipoxygenases; EC 1.13.11.12; 9029-60-1; 13-lipoxidase; carotene oxidase; 13-lipoperoxidase; fat oxidase; 13-lipoxydase; lionoleate:O2 13-oxidoreductase; linoleate 13S-lipoxygenase
