Cat No.
NATE-0446
Description
Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.
Abbr
MDH, Native (Chicken)
Alias
malic enzyme
Source
Chicken liver
Species
Chicken
Form
ammonium sulfate suspension; Suspension in 2.9 M (NH4)2SO4 solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na4EDTA, pH 6.0
Enzyme Commission Number
EC 1.1.1.40
Bio-activity
10-30 units/mg protein (modified Warburg-Christian)
CAS No.
9028-47-1
Unit Definition
One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO2, and NADPH per min at pH 7.4 at 25°C.
Applications
Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.
Synonyms
malic enzyme (ambiguous); pyruvic-malic carboxylase (ambiguous); malate dehydrogenase (decarboxylating, NADP+); NADP+-linked decarboxylating malic enzyme; NADP+-malic enzyme; NADP+-specific malic enzyme; NADP-specific malate dehydrogenase; malate dehydrogenase (NADP+, decarboxylating); L-malate:NADP+oxidoreductase; EC 1.1.1.40; 9028-47-1
