Cat No.
NATE-1100
Description
In enzymology, an inositol 2-dehydrogenase (EC 1.1.1.18) is an enzyme that catalyzes the chemical reaction:myo-inositol + NAD+? 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+. Thus, the two substrates of this enzyme are myo-inositol and NAD+, whereas its 3 products are 2,4,6/3,5 pentahydroxycyclohexanone, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in inositol metabolism and inositol phosphate metabolism.
Abbr
myo-Inositol Dehydrogenase, Recombinant (B. subtilis)
Species
Bacillus subtilis
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 1.1.1.18
Bio-activity
67.5 U/mg protein at pH 9.6 and 25°C.
Molecular Mass
~ 39,417
Isoelectric point
~ 5.3
Unit Definition
One Unit of myo-inositol dehydrogenase is defined as the amount of enzyme required to produce one μmole of scyllo-inosose and NADH from myo-inositol and NAD+ per minute at 25°C.
Optimum pH
9.6
Thermal stability
up to 25°C
Stability
> 2 years at 4°C
Preparation Instructions
For assay, this enzyme should be diluted in assay buffer. Swirl to mix the enzyme suspension immediately prior to use.
Applications
High purity recombinant myo-Inositol dehydrogenase (B. subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
myo-inositol 2-dehydrogenase; myo-inositol:NAD+ oxidoreductase; inositol dehydrogenase; myo-inositol dehydrogenase; EC 1.1.1.18; 9028-25-5
