Cat No.
NATE-1116
Description
The presumed function of NADH peroxidase is to inactivate H2O2 generated within the cell, for example by glycerol-3-phosphate oxidase during glycerol metabolism or dismutation of superoxide, before the H2O2 causes damage to essential cellular components.
Abbr
NADH Peroxidase, Recombinant (E. faecalis)
Species
Enterococcus faecalis
Form
A solution containing 50% glycerol plus 0.1 mg/mL FAD and 0.02% (w/v) sodium azide.
Enzyme Commission Number
EC 1.11.1.1
Bio-activity
59 U/mg protein at pH 5.5 and 25°C.
Molecular Mass
~ 50,388
pH Stability
6.0 - 9.0
Isoelectric point
~ 5.1
Unit Definition
One Unit of NADH peroxidase is defined as the amount of enzyme required to oxidise one μmole of NADH to NAD+ per minute.
Optimum pH
5.0-6.0
Optimum temperature
37°C
Thermal stability
up to 40°C
Stability
> 2 years at -20°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 5.5 containing 0.5 mg/mL BSA.
Applications
High purity recombinant NADH peroxidase (E. faecalis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
NADH:hydrogen-peroxide oxidoreductase; DPNH peroxidase; NAD peroxidase; diphosphopyridine nucleotide peroxidase; NADH-peroxidase; nicotinamide adenine dinucleotide peroxidase; NADH2 peroxidase; EC 1.11.1.1
