Cat No.
NATE-0488
Description
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical. Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.
Abbr
Nitroreductase, Recombinant (E. coli)
Source
E. coli
Species
Escherichia coli
Molecular Mass
monomer mol wt 24 kDa
Purification
> 90% (SDS-PAGE)
Unit Definition
One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37°C.
Applications
Nitroreductase has been used in a study that used a set of PCR primers to clone a gene encoding a hypothetical nitroreductases (named as Ssap-NtrB) from uropathogenic staphyl oc occus. It has also been used to improve prodrug activation. Nitroreductase from Escherichia coli has been used in a study to assess anaerobic bacteria as a gene delivery system for cancer treatment. It has also been used in a study to investigate its applications in antibody-directed enzyme prodrug therapy.
Warnings
Produced using animal component-free materials.
Gene Name
nfsA nitroreductase A, NADPH-dependent, FMN-dependent [Escherichia coli str. K-12 substr. MG1655]
Synonyms
Nitroreductase
GeneID
945483
Protein Refseq
NP_415372
UniProt ID
P17117
Pathway
Microbial metabolism in diverse environments, organism-specific biosystem; Nitrotoluene degradation, organism-specific biosystem; Nitrotoluene degradation, conserved biosystem
