Cat No.
NATE-0500
Description
Pyranose oxidase (P2O) catalyzes the oxidation of aldopyranoses at position C-2 to yield the corresponding 2-ketoaldoses. P2O is a homotetrameric protein that contains covalently bound flavin adenine dinucleotide (FAD). The in vivo substrates of P2O are thought to be D-glucose, D-galactose, and D-xylose. They are oxidized to 2-keto-D-glucose (D-arabino-hexos-2-ulose, 2-dehydro-D-glucose), 2-keto-D-galactose (D-lyxo-hexos-2-ulose, 2-dehydro-D-galactose), and 2-keto-D-xylose (D-threopentos-2-ulose, 2-dehydro-D-xylose), respectively. Pyranose oxidase has significant activity with carbohydrates such as, L-sorbose, D-glucono-1,5-lactone, and D-allose. When pyranose oxidase catalyzes the oxidation of aldopyranoses, electrons are transferred to molecular oxygen which results in the formation of hydrogen peroxide.
Abbr
P2O, Recombinant (Coriolus sp.)
Alias
P2O
Source
E. coli
Species
Coriolus sp.
Enzyme Commission Number
EC 1.1.3.10
Bio-activity
> 2.7 units/mg solid
CAS No.
37250-80-9
Unit Definition
One unit produces 1.0 μmol of hydrogen peroxide per minute at 37°C, pH 7.0.
Applications
Pyranose oxidase (P20) is used for the determination of D-glucose and 1,5-anhydroglucitol in clinical analysis. It is used to study the biotransformations of carbohydrates and is used as an important marker for glycemic control in diabetes patients
Warnings
Contains glutamate
Synonyms
pyranose oxidase; EC 1.1.3.10; glucose 2-oxidase; pyranose-2-oxidase; 37250-80-9; P2O
