Cat No.
NATE-0509
Description
In enzymology, a protocatechuate 3,4-dioxygenase (EC 1.13.11.3) is an enzyme that catalyzes the chemical reaction:3,4-dihydroxybenzoate + O2? 3-carboxy-cis,cis-muconate. Thus, the two substrates of this enzyme are 3,4-dihydroxybenzoate (protocatechuic acid) and O2, whereas its product is 3-carboxy-cis,cis-muconate. This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O2 as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, iron.
Abbr
PCD, Native (Pseudomonas sp.)
Alias
PCD
Species
Pseudomonas sp.
Form
lyophilized powder; Supplied as lyophilized powder containing approx. 40% stabilizer.
Enzyme Commission Number
EC 1.13.11.3
Bio-activity
> 3 units/mg solid
CAS No.
9029-47-4
Molecular Mass
mol wt ~700 kDa
pH Stability
pH 7.0?9.0 (25°C, 72hr)
Structure
Protein with nonheme iron
Unit Definition
One unit will oxidize 1.0 μmole of protocatechuate to 3-carboxy-cis,cis-muconate per min at pH 7.5 at 37°C.
Optimum pH
9.0
Optimum temperature
60?65°C
Thermal stability
below 50°C (pH 6.0, 1hr)
Inhibitors
Ag+, Hg++, PCMB
Applications
Prot ocatechuate 3,4-Dioxygenase (PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of prot ocatechuate in Rhizobiaceae. The enzyme has been used to create an oxygen scavenging system along with prot ocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and prot ocatechuate in single molecule motility assay.
Warnings
Protein determined by biuret.
Synonyms
protocatechuate 3,4-dioxygenase; protocatechuate oxygenase; protocatechuic acid oxidase; protocatechuic 3,4-dioxygenase; protocatechuic 3,4-oxygenase; 9029-47-4; EC 1.13.11.3; PCD
