Cat No.
NATE-0550
Description
Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form:ROOR' + electron donor (2 e?) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.
Abbr
Peroxidase, Native (Horseradish)
Alias
Peroxidase
Source
Horseradish Roots
Species
Horseradish
Form
A soluble , dialyzed, lyophilized powder
Enzyme Commission Number
EC 1.11.1.7
Bio-activity
> 85 units per mg dry weight
Molecular Mass
40 kDa (Maehly 1955).
Composition
Seven isozymes have been described by Shannon et al. (1966);Kay et al. (1967); and Strickland et al. (1968). See also Delincée and Radola (1975) and Shih et al. (1971). All contain photohemin IX as prosthetic group. Neutral and amino sugars account for approximately 18% of the enzyme. Weinryb (1966) indicates that the "active site" involves apoprotein as well as the heme group. See also Lanir and Schejter (1975). Dolman et al. (1975) have reported on the formation of Compound I. See also Dunford et al. (1975), Santimone (1975) and Stillman et al. (1975).
Isoelectric point
7.2 (Maehly 1955).
Specificity
The enzyme exhibits a high specificity. Activity is observed with H2O2, MeOOH, and EtOOH (Maehly and Chance 1954). See also Chmielnicka et al. (1971) and Morrison and Bayse (1973).
Unit Definition
One Unit decomposes 1μmole of H2O2 per minute at 25°C, pH 7.0 using aminoantipyrine and phenol.
Optimum pH
7.0 (Maehly 1955).
Stability
The enzyme is quite stable. As a lyophilized powder, it may be stored several years refrigerated.
Inhibitors
Horseradish peroxidase is reversibly inhibited by cyanide and sulfide at a concentration of 10-5 M (Theorell 1951).
Gene Name
MPO myeloperoxidase [Homo sapiens]
Synonyms
EC 1.11.1.7; Peroxidase; lactoperoxidase; guaiacol peroxidase; plant peroxidase; Japanese radish peroxidase; horseradish peroxidase (HRP); soybean peroxidase (SBP); extensin peroxidase; heme peroxidase; oxyperoxidase; protoheme peroxidase; pyrocatechol peroxidase; scopoletin peroxidase; Coprinus cinereus peroxidase; Arthromyces ramosus peroxidase
GeneID
4353
mRNA Refseq
NM_000250
Protein Refseq
NP_000241
MIM
606989
UniProt ID
P05164
Chromosome Location
17q21.3-q23
Pathway
C-MYB transcription factor network, organism-specific biosystem; Folate Metabolism, organism-specific biosystem; IL23-mediated signaling events, organism-specific biosystem; Phagosome, organism-specific biosystem; Phagosome, conserved biosystem; Selenium Pathway, organism-specific biosystem; Transcriptional misregulation in cancer, organism-specific biosystem
Function
chromatin binding; heme binding; heparin binding; metal ion binding; oxidoreductase activity; peroxidase activity
