Cat No.
NATE-0678
Description
Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. SOD plays a critical role in the defense of cells against the toxic effects of oxygen radicals. SOD competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice by preventing the conversion of NO to peroxynitrate, an inducer of apoptosis.
Abbr
SOD, Native (E. coli)
Alias
SOD; SODF; SOD-1
Species
Escherichia coli
Form
Lyophilized powder containing Tris buffer salts
Enzyme Commission Number
EC 1.15.1.1
Bio-activity
> 1 ,000 units/mg protein
CAS No.
9054-89-1
Molecular Mass
mol wt 32.5 kDa
Unit Definition
One unit will inhibit reduction of Cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25°C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
Applications
The Cu,Zn superoxide dismutase from Escherichia coli retains monomeric structure at high protein concentration, which could be applied towards future assessments of altered subunit interaction in all the bacteri ocupreins. Superoxide dismutase from Escherichia coli has also been used in a study to investigate the superoxide anion participattion in 2-oxoglutarate-dependent hydroxylation.
Warnings
Iron-containing enzyme; For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).
Gene Name
sodA superoxide dismutase [Escherichia coli CFT073]; sodB superoxide dismutase [Escherichia coli CFT073]; sodC superoxide dismutase [Escherichia coli CFT073]
Synonyms
Superoxide dismutases; EC 1.15.1.1; superoxidase dismutase; copper-zinc superoxide dismutase; Cu-Zn superoxide dismutase; ferrisuperoxide dismutase; superoxide dismutase I; superoxide dismutase II; SOD; Cu,Zn-SOD; Mn-SOD; Fe-SOD; SODF; SODS; SOD-1; SOD-2; SOD-3; SOD-4; hemocuprein; erythrocuprein; cytocuprein; cuprein ; hepatocuprein; 9054-89-1
GeneID
1040208; 1036179; 1036143
Protein Refseq
NP_756713; NP_753945; NP_753933
UniProt ID
P0AGD4
