Cat No.
NATE-0676
Description
Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. SOD plays a critical role in the defense of cells against the toxic effects of oxygen radicals. SOD competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice by preventing the conversion of NO to peroxynitrate, an inducer of apoptosis.
Abbr
SOD, Native (Bovine)
Alias
SOD; SODF; SOD-1
Source
Bovine liver
Species
Bovine
Form
Type I, Lyophilized powder containing potassium phosphate buffer salts; Type II, ammonium sulfate suspension, Suspension in 3.8 M (NH4)2SO4, pH 7.0.
Enzyme Commission Number
EC 1.15.1.1
Bio-activity
Type I, > 1500 units/mg protein; Type II, 2 ,000-6 ,000 units/mg protein (biuret).
CAS No.
9054-89-1
Molecular Mass
mol wt 32.5 kDa
Unit Definition
One unit will inhibit reduction of Cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25°C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
Applications
Superoxide dismutase from bovine liver has been used in a study to determine that hypercholesterolemia increases endothelial superoxide anion production. Superoxide dismutase from bovine liver has also been used in a study to investigate diazo coupling, subunit interactions and electrophoretic variants of bovine erythr ocyte superoxide dismutase.
Warnings
For assay method, see McCord, J.M. and Fridovich, I., J. Biol. Chem., 244, 6049 (1969).
Gene Name
SOD1 superoxide dismutase 1, soluble [Bos taurus]
Synonyms
Superoxide dismutases; EC 1.15.1.1; superoxidase dismutase; copper-zinc superoxide dismutase; Cu-Zn superoxide dismutase; ferrisuperoxide dismutase; superoxide dismutase I; superoxide dismutase II; SOD; Cu,Zn-SOD; Mn-SOD; Fe-SOD; SODF; SODS; SOD-1; SOD-2; SOD-3; SOD-4; hemocuprein; erythrocuprein; cytocuprein; cuprein ; hepatocuprein; 9054-89-1
GeneID
281495
mRNA Refseq
NM_174615
Protein Refseq
NP_777040
UniProt ID
P00442
Chromosome Location
1q12-q14
Function
chaperone binding; copper ion binding; ubiquitin-protein transferase activity
