Cat No.
NATE-0681
Description
Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. SOD plays a critical role in the defense of cells against the toxic effects of oxygen radicals. SOD competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice by preventing the conversion of NO to peroxynitrate, an inducer of apoptosis.
Abbr
SOD, Recombinant (Bovine)
Alias
SOD; SODF; SOD-1
Source
E. coli
Species
Bovine
Form
lyophilized powder
Enzyme Commission Number
EC 1.15.1.1
Bio-activity
> 2500 units/mg protein
CAS No.
9054-89-1
Purification
> 90% (SDS-PAGE)
pH Stability
7.6-10.5
Isoelectric point
4.95
Unit Definition
One unit will inhibit reduction of Cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25°C in a 3.0 ml reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
Optimum pH
7.8 (25°C)
Inhibitors
cyanide, OH-(competitive), hydrogen peroxide
Applications
Superoxide dismutase has been used in a study to investigate where lipoproteins may affect the L-arginine-nitric oxide pathway. Superoxide dismutase has also been used in a study to investigate the mass spectral evidence for carbonate-anion-radical-induced posttranslational modification of tryptophan to kynurenine in human Cu, Zn superoxide dismutase. The product has been used to develop an SOD assay. This assay used dismutase-mediated inhibition of NADH-dependent nitroblue tetrazolium reduction.
Warnings
Produced using animal component-free materials. Extinction coefficient: EmM= 10.3 (258 nM). SOD has no significant absorbance peak at 280 nM because of the absence of tryptophan.
Gene Name
SOD1 superoxide dismutase 1, soluble [Bos taurus]
Synonyms
Superoxide dismutases; EC 1.15.1.1; superoxidase dismutase; copper-zinc superoxide dismutase; Cu-Zn superoxide dismutase; ferrisuperoxide dismutase; superoxide dismutase I; superoxide dismutase II; SOD; Cu,Zn-SOD; Mn-SOD; Fe-SOD; SODF; SODS; SOD-1; SOD-2; SOD-3; SOD-4; hemocuprein; erythrocuprein; cytocuprein; cuprein ; hepatocuprein; 9054-89-1
GeneID
281495
mRNA Refseq
NM_174615
Protein Refseq
NP_777040
UniProt ID
P00442
Chromosome Location
1q12-q14
Function
chaperone binding; copper ion binding; ubiquitin-protein transferase activity
