Cat No.
NATE-0713
Description
Thioredoxin reductase (TrxR) is an NADPH-dependent oxidoreductase containing one FAD per subunit that reduces the active site disulfide in oxidised thioredoxin (Trx). The molecular weight of the isozymes from mammalian sources vary between 55-67 kDa as compared with 35 kDa in prokaryotes, plants or yeast. The substrate specificity of the mammalian enzyme is much broader than the prokaryotic enzyme reducing both mammalian and E. coli thioredoxins as well as well as non-disulfide substrates such selenite, lipoic acids, lipid hydroperoxides and hydrogen peroxide.
Abbr
TR, Native (Rat)
Alias
TrxR
Source
Rat liver
Species
Rat
Form
buffered aqueous glycerol solution; Solution in 50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 1 mM EDTA, and 10% glycerol.
Enzyme Commission Number
EC 1.8.1.9
Bio-activity
> 100 units/mg protein (Bradford)
CAS No.
9074-14-0
Unit Definition
One unit will cause an increase in absorbance of 1.0 at 412 nm (when measured in a non-coupled assay containing DTNB alone as substrate) per minute at pH 7.0 at 25°C.
Applications
Thioredoxin Reductase from rat liver can be used for studying the uptake and reduction of a-lipoic acid by utilizing reducing capacity of human erythr ocytes. The product can also be used for studying the activation mechanism of transglutaminase 2 (TG2) in the extracellular matrix by using Thioredoxin.
Gene Name
TXNRD1 thioredoxin reductase 1 [Homo sapiens]
Synonyms
NADP-thioredoxin reductase; NADPH-thioredoxin reductase; thioredoxin reductase (NADPH); NADPH2:oxidized thioredoxin oxidoreductase; thioredoxin-disulfide reductase; EC 1.8.1.9; TrxR; 9074-14-0
GeneID
7296
mRNA Refseq
NM_001093771
Protein Refseq
NP_001087240
MIM
601112
UniProt ID
Q16881
Chromosome Location
12q23-q24.1
Pathway
Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Oxidative Stress, organism-specific biosystem; PPARA Activates Gene Expression, organism-specific biosystem; Pyrimidine metabolism, organism-specific biosystem
Function
NADP binding; electron carrier activity; flavin adenine dinucleotide binding; oxidoreductase activity; protein disulfide oxidoreductase activity; thioredoxin-disulfide reductase activity
