Cat No.
NATE-1065
Description
Xanthine oxidoreductase (XOR) catalyzes the formation of uric acid from hypoxanthine and xanthine, last two steps of purine catabolism. The mammalian enzyme is synthesized as a xanthine dehydrogenase form (XDH, EC 1.17.1.4), which uses NAD as the electron acceptor, but is converted into an xanthine oxidase form (XO, EC 1.1.3.22) by reversible (through sulfhydryl group oxidation) or irreversible (proteolysis) manner. Since most industrial protocols of XOR purification includes proteolysis step, commercial XOR enzyme is available only as oxidase form unable to use NAD as a an electron acceptor.
Abbr
XDH, Native (Bovine milk)
Alias
XDH
Source
Bovine milk
Species
Bovine
Form
Lyophilized
Enzyme Commission Number
EC 1.17.1.4
Unit Definition
One unit of XDH catalyzes the reduction of 1μmol of NAD per minute at pH 8.8 with concomitant oxidation of hypoxanthine to uric acid.
Synonyms
xanthine dehydrogenase; NAD+-xanthine dehydrogenase; xanthine-NAD+ oxidoreductase; xanthine/NAD+ oxidoreductase; xanthine oxidoreductase; XDH; EC 1.17.1.4
