Cat No.
NATE-0773
Description
In enzymology, a DNA beta-glucosyltransferase is an enzyme that catalyzes the chemical reaction in which a beta-D-glucosyl residue is transferred from UDP-glucose to an hydroxymethylcytosine residue in DNA. It is analogous to the enzyme DNA alpha-glucosyltransferase. This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases.
Abbr
β-glucosyltransferase, Recombinant
Source
E. coli
Form
aqueous solution, Formulated in 200 mM imidazole and 20% glycerol.
Molecular Mass
mol wt 41.6 kDa
Purification
> 83% (SDS-PAGE)
Applications
Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.
Gene Name
b-gt adds a glucose in beta linkage to hydroxymethylcytosine residues in phage DNA [Enterobacteria phage T4]
Synonyms
T4 Beta-glucosyltransferase; DNA beta-glucosyltransferase; T4-HMC-beta-glucosyl transferase; T4-beta-glucosyl transferase; T4 phage beta-glucosyltransferase; UDP glucose-DNA beta-glucosyltransferase; uridine diphosphoglucose-deoxyribonucleate beta-glucosyltransferase
GeneID
1258765
UniProt ID
P04547
