Cat No.
NATE-1095
Description
Alanine transaminase (ALT) is a transaminase enzyme. It is also called alanine aminotransferase (ALAT) and was formerly called serum glutamate-pyruvate transaminase (SGPT) or serum glutamic-pyruvic transaminase (SGPT). ALT is found in plasma and in various body tissues, but is most common in the liver. It catalyzes the two parts of the alanine cycle. Serum ALT level, serum AST (aspartate transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels.
Abbr
ALT, Recombinant (B. subtilis)
Alias
ALT; ALAT; GPT
Species
B. subtilis
Form
Ammonium sulphate suspension.
Enzyme Commission Number
EC 2.6.1.21
Bio-activity
66 U/mg protein at pH 7.5 and 25°C.
Concentration
2,000 U/mL
Molecular Mass
~ 33,346
Isoelectric point
~ 5.7
Unit Definition
One Unit of D-glutamate-pyruvate transaminase (D-GPT) is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH.
Optimum pH
7.5
Thermal stability
up to 25°C
Stability
> 4 years at 4°C
Preparation Instructions
Swirl to mix the enzyme suspension immediately prior to use.
Applications
High purity D-Glutamate-pyruvate transaminase (B. subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
ALT1; Glutamic-pyruvic transaminase 1; GPT 1; Glutamic-alanine transaminase 1; AAT1; ALT; ALAT; SGPT; Alanine transaminase; alanine aminotransferase; GPT; β-alanine aminotransferase; alanine-α-ketoglutarate aminotransferase; alanine-pyruvate aminotransferase; glutamic acid-pyruvic acid transaminase; glutamic-pyruvic aminotransferase; L-alanine aminotransferase; L-alanine transaminase; L-alanine-α-ketoglutarate aminotransferase; pyruvate transaminase; pyruvate-alanine aminotransferase; pyruvate-glutamate transaminase
