Cat No.
NATE-1271
Description
The Casein kinase 2 (EC 2.7.11.1) is a serine/threonine-selective protein kinase that is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm and other cellular processes.
Abbr
CK II, Recombinant (Human)
Alias
CK II
Source
E. coli
Species
Human
Form
350 mM NaCl, 20 mM Tris-HCl (pH 7.5 25°C), 1 mM Na2EDTA, 2 mM DTT and 0.1% Triton X-100.
Bio-activity
859,000 units/mg
Concentration
500,000 units/ml
Molecular Mass
a-subunit (45 kDa), b-subunit (25 kDa). The apparent molecular weight of the a-subunit estimated by SDS-PAGE is about 42 kDa.
Unit Definition
One unit is defined as the amount of CK2 required to catalyze the transfer of 1 pmol of phosphate to CK2 Peptide Substrate, RRRADDSDDDDD (100 μM), in 1 minute at 30°C in a total reaction volume of 25 μl.
Synonyms
EC 2.7.11.1; Casein Kinase II; CK2; CK II; Casein Kinase 2
