Cat No.
NATE-0155
Description
Cytosolic protamine kinase is involved in the regulation of protein synthesis and is indirectly associated with numerous cellular processes. Cytosolic protamine kinase is a distinct insulin-stimulated kinase involved in the phosphorylation of eukaryotic Initiation Factor 4E (eIF4E) which is key to initiating translation by mRNA. This protein appears to be inactivated by protein phosphatase 2A family members and may also be inhibited by microcystin, okadeic acid, and ATP. The phosphorylation process is reversible and MBPK1 and MBPK2 (Myelin Basic Protein Kinase 1 and 2) may reactivate cytosolic protamine kinase.
Abbr
CPK, Native (Bovine)
Alias
CPK; Cpk
Source
Bovine kidney
Species
Bovine
Form
buffered aqueous glycerol solution
Bio-activity
> 15,000 units/mg protein
Purification
>90% (SDS-PAGE)
Unit Definition
One unit will incorporate 1 nmol of phosphate using protamine sulfate as a substrate per minute at pH 7.0 at 30°C.
Applications
Cytosolic Protamine Kinase (cPK) is isolated from bovine kidney and has a molecular mass of approximately 45 kDa. It phosphorylates the eukaryotic Initiation Factor 4E (eIF4E), which initiates translation by mRNA. It is used to study protein synthesis and various cellular processes.
Synonyms
Protamine Kinase, Cytosolic; Cytosolic protamine kinase; CPK; Cpk
