Cat No.
NATE-0824
Description
(d)CMP kinase (EC 2.7.4.25, prokaryotic cytidylate kinase, deoxycytidylate kinase, dCMP kinase, deoxycytidine monophosphokinase) is an enzyme with system name ATP:(d)CMP phosphotransferase. This enzyme catalyses the following chemical reaction: ATP + (d)CMP ? ADP + (d)CDP. The prokaryotic cytidine monophosphate kinase specifically phosphorylates CMP (or dCMP).
Abbr
dCMP kinase, Recombinant (Prokaryote)
Species
Prokaryote
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 2.7.4.25
Bio-activity
17 U/mg protein at pH 7.6 and 25°C.
Molecular Mass
~ 26,910 Da
Isoelectric point
~ 6.2
Unit Definition
One Unit of cytidylate kinase is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH.
Optimum pH
7.6
Optimum temperature
25°C
Stability
> 2 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in 100 mM TEA buffer, pH 7.6 containing 1 mg/ml BSA. Swirl to mix the enzyme suspension immediately prior to use.
Applications
High purity recombinant Cytidylate kinase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
Cytidylate kinase; (d)CMP kinase; EC 2.7.4.25; prokaryotic cytidylate kinase; deoxycytidylate kinase; dCMP kinase; deoxycytidine monophosphokinase
